منابع مشابه
PCR amplification of long DNA fragments.
The polymerase chain reaction (PCR) has recently evolved as a standard laboratory technique, popular in all areas of molecular biology research. However, the technique still has two limitations: the relatively low fidelity of Taq polymerase when compared with other polymerases (1), and its inability to efficiently amplify fragments higher than 3 kbp (2, 3). Although these two issues are irrelev...
متن کاملSize-selective separation and overall-amplification of cell-free fetal DNA fragments using PCR-based enrichment
This study aimed to establish a method for the selective amplification of cell-free fetal DNA (cffDNA) in maternal plasma and preserve the integrity of DNA fragments during amplification, thereby providing a sufficient amount of cffDNA to meet the requirement of routine non-invasive prenatal testing. We amplified DNA molecules in a one-reaction system without considering their particular sequen...
متن کاملLong-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus
DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex. We report here the assembly of the functional holoenzyme from Thermus thermophilus (Tth), an extreme thermophile. The minimal h...
متن کاملSpermidine facilitates PCR amplification of target DNA.
Department of Agronomy and Range Science, University of California, Davis, California 95616-8515 Recent advances in PCR have made this technique one of the most powerful tools for a wide spectrum of molecular analyses, such as genome mapping, molecular evolution, diagnosis of genetic disease, and forensic sciences. ~ Many PCR applications involve the specific and reproducible amplif icat ion of...
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1.Belgrader, P., W. Benett, D. Hadley, G. Long, R. Mariella, Jr., F. Milanovich, S. Nasarabadi, W. Nelson et al. 1998. Rapid pathogen detection using a microchip PCR array instrument. Clin. Chem. 44:2191-2194. 2.Belgrader, P., J.K. Smith, V.W. Weedn and M.A. Northrup. 1998. Rapid PCR identity testing using a battery-powered miniature thermal cycler. J. Forensic Sci. 43:315-319. 3.Belinda, A.J.G...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1992
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/20.3.623